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| Registros recuperados: 858 | |
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| Soletchnik, Patrick; Goulletquer, Philippe; Razet, Daniel; Geairon, Philippe; Le Moine, Olivier; Faury, Nicole; Turpin, Vincent; Hamon, R.; Robert, Jean-michel; Hussenot, Jerome; Brossard, Nicolas. |
| Cette étude constitue une des trois actions engagées dans le cadre du Contrat de Plan IFREMER - Poitou-Charentes (1994-1998) sous l"intitulé Valorisation des Claires Ostréicoles. Les deux actions complémentaires concernent la compréhension et l'amélioration du verdissement des claires sous la direction du Pr. J.M. Robert de l'Université de Nantes/lSOMER et la production de masse de microalgues afin de stimuler la croissance de mollusques filtreurs sous la direction de Dr. J. Hussenot du CREMA L'Houmeau. Ce programme répond directement à la demande des professionnels afin d'optimiser les conditions d'élevage de l'huître creuse C. gigas en période d'affinage qui à l'heure actuelle, ne permettent pas le contrôle de la qualité finale du produit. L'objectif... |
| Tipo: Text |
Palavras-chave: Claires ostréicoles; Affinage; Capacité trophique; Paramètres; Marais expérimental; Culture diatomée; Huître creuse; Crassostrea gigas; Haslea ostrearia. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00044/15541/12918.pdf |
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| Huvet, Arnaud; Boudry, Pierre; Ohresser, M; Delsert, Claude; Bonhomme, F. |
| Source/description : Genomic DNA was extracted from a whole Pacific oyster (C. gigas) after grinding in liquid nitrogen. Purified DNA was then digested by a mix of 3 restriction enzymes (AluI, RsaI, HaeIII). DNA fragments ranging from 250 to 500 bp were size-selected by agarose gel electrophoresis and ligated into the dephosphorylated blunt-ended SmaI-linearised pBKS2 plasmid (Stratagene Cloning System, CA). White colonies (11,500) were screened by hybridisation with double-stranded DNA probes containing dinucleotide repeats. Poly(dAdC), poly(dAdG), poly(dAdT) (Pharmacia Biotech, Sweden) were mixed at equimolar ratio and radiolabelled by [-32P] alpha dATP and dCTP using a random priming labelling kit (Life Technologies, Germany). Filter... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Oyster; Microsatellites. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-703.pdf |
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| Gras, Marie-paule; Gras, Paul. |
| At the beginning of its introduction, following the massive mortality rate of 1970-1971 that struck the Portuguese oyster, Crassostrea angulata (COMPS and GRAS 1973), the oyster from the Pacific, Crassostrea gigas, displayed excellent growth in addition to exceptional hardiness and resistance to mortality. But, since 1973, in most of the farming areas, there has been a slowdown in the growth of the oysters and an at times quite pronounced decrease in their quality. It was in order to better understand their adaptation to the environment and the causes of the lowering of their growth rate and their condition that a study was undertaken in 1974, in one of the largest areas of flat cultivation of the Marennes-Oléron basin, located on the east coast of the... |
| Tipo: Text |
Palavras-chave: Marennes Oléron; Biochimie; Biométrie; Huîtres; Crassostrea gigas. |
| Ano: 1975 |
URL: http://archimer.ifremer.fr/doc/1975/acte-2988.pdf |
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| Labreuche, Yannick; Le Roux, Frederique; Henry, Joel; Zatylny, Celine; Huvet, Arnaud; Lambert, Christophe; Soudant, Philippe; Mazel, Didier; Nicolas, Jean-louis. |
| Extracellular products (ECPs) of the pathogenic Vibrio aestuarianus 01/32 were previously reported to display lethality in Crassostrea gigas oysters and to cause morphological changes and immunosuppression in oyster hemocytes. To identify the source of this toxicity, biochemical and genetic approaches were developed. ECP protease activity and lethality were shown to be significantly reduced following incubation with metal chelators, suggesting the involvement of a zinc metalloprotease. An open reading frame of 1836 bp encoding a 611-aa metalloprotease (designated yam) was identified. The deduced protein sequence showed high homology to other Vibrio metalloproteases reported to be involved in pathogenicity. To further confirm the role of this enzyme in ECP... |
| Tipo: Text |
Palavras-chave: Vibrio aestuarianus; Metalloprotease; Crassostrea gigas; Oyster; Hemocytes; Extracellular products. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00015/12649/9588.pdf |
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| Le Roux, Frederique; Goubet, A; Thompson, F; Faury, Nicole; Gay, Melanie; Swings, J; Saulnier, Denis. |
| Polyphasic analysis of four new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on DNA gyrase subunit B (gyrB), RNA polymerase Quo factor (rpoD), replication origin-binding protein (rctB) and transmembrane regulatory protein (toxR) genes, fluorescent amplified fragment length polymorphism and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed that these new isolates should be accommodated in a novel species, Vibrio gigantis sp. nov. Phenotypic features that... |
| Tipo: Text |
Palavras-chave: Vibrio gigantis; Haemoplymph; Disease; Crassostrea gigas; Oyster culture. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-792.pdf |
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| Boher, S; Piclet, Guy; Beril-stien, S; Masson, Daniel; Delonce, R; Biziagos, E; Schwartzbrod, L. |
| Shellfish consumption is clearly associated with the transmission of enteric viruses. The channel of transmission of enteric viruses to human beings could be decreased particularly if the systems of depuration of shellfish were optimized, standardized and if their viral efficiency was controlled. The purpose of this work was to study the contamination of oysters and to assess the efficiency of several depuration processes. Our study have demonstrated that in some cases, 50% of oysters samples contain enteric viruses or H.A.V. antigen and the quantities of viruses range from 17 to 246 FF for the rotaviruses and from 124 to 200 for the enteroviruses per 100 g of tissue. According to our results, the exclusive use of a bacteriological criterium to determine... |
| Tipo: Text |
Palavras-chave: Rotavirus; Hepatitis A virus; Enterovirus; Crassostrea gigas; Bivalvia; Seafood; Bioassays; Cell culture; Viruses; Self purification; Viral diseases; Shellfish. |
| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/acte-1615.pdf |
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| Garcia, Celine; Robert, Maeva; Arzul, Isabelle; Chollet, Bruno; Joly, Jean-pierre; Miossec, Laurence; Comtet, Thierry; Berthe, Franck. |
| Viral gametocytic hypertrophy was reported for the first time in 2001 in Pacific oyster Crassostrea gigas in France. Since this date, the number of reported cases and the distribution area have increased every year; however, the cases are not associated with macroscopic signs or increased mortality rates. Both male and female gametes were hypertrophied and basophilic inclusions were observed in gamete nuclei, Transmission electron microscopy revealed the presence of viral particles in these intranuclear basophilic inclusions. These particles had characteristics similar to those of the Papillomaviridae and Polyomaviridae families: they were small, non-enveloped, icosahedral, and 44 to 56 nm in diameter, The viral particles were found in male, female and... |
| Tipo: Text |
Palavras-chave: Polyomaviridae; Papillomaviridae; Gonad; Viral garnetocytic hypertrophy; Pacific oyster; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-3288.pdf |
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| Riera, Pascal. |
| This study examined the delta(15)N of suspended and sedimented particulate organic matter, primary sources of organic matter and benthic invertebrates along an estuarine gradient in Marennes-Oleron Bay, France. Particular emphasis was given to the use of delta(15)N as a tracer of the origin of organic matter and as a means of determining the benthic food web structure in estuarine environments. delta(15)N values indicated that there was direct utilisation of benthic diatoms as a food source by oyster Crassostrea gigas near intertidal mudflats but suggested trophic mediation between terrestrial detritus and oysters in the upper estuarine reaches. delta(15)N data showed that the trophic position of oysters may vary in the estuarine bay. The delta(15)N values... |
| Tipo: Text |
Palavras-chave: Nitrogen isotope ratio; Particulate organic matter; Crassostrea gigas; Benthic food web; Estuary; Marennes-Oleron Bay. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00448/55926/57786.pdf |
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| Registros recuperados: 858 | |
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